Nanoscopy Netherlands 2014

Title: Nanoscopy Netherlands 2014
Date: 29-30 January 2014
Location: FNWI, University of Amsterdam, Amsterdam
Organiser: Erik Manders
Sponsor: Nikon Instruments

Introduction

Two years ago a large-scale program on the development of super-resolution technology has started in The Netherlands. This national program has been granted by Technology Foundation STW (STW-perspectief) to a consortium of scientists and companies to make nanoscopy broadly applicable in biomedical research.

In October 2012, now one year ago, we opened in Amsterdam the Nikon Centre of Excellence on Super Resolution Microscopy Development, a collaborative centre of the Van Leeuwenhoek Centre for Advanced Microscopy of the University of Amsterdam and Nikon Instruments Europe. Apart from a scientific collaboration on development of new technology, we organize trainings and courses. In addition, we will organize symposia on super-resolution microscopy technology and its biological applications. The organisation of this symposium was an initiative within the Centre of Excellence and Nikon decided to fully sponsor this event which allowed us to invite excellent speakers.

We invite you to come to this symposium in super-resolution microscopy and its applications in biomedical sciences. We have found a large number of excellent speakers who will explain the basic techniques and show their most recent results. In addition some of the members of the nanoscopy consortium will show the half-way results of the program.

This symposium will be a interesting for scientists ranging from the just-starting microscopists to experts in super-resolution microscopy, ranging from biologists interested in new technology to physicists interested in biological and ranging from just-started master-students to most-experienced professors.
We welcome you to “Nanoscopy Netherlands 2014”.
Erik Manders

Program

During the symposium all the current super-resolution microscopy techniques will be discussed. On Wednesday morning (January 29) we will start with some introductory lectures and in the afternoon Structured Illumination Microscopy and related techniques will be the topic. On Thursday (January 30) the main topic will be localisation microscopy techniques like PALM, STORM and GSDIM but also molecule tracking, new fluorescent molecules and STED microscopy will be discussed.

Venue: Room C1.110, Science Park 904, Amsterdam, The Netherlands

Wednesday, January 29, 2014

10:00 – 11:00 Registration and coffee in main hall of Science Park

Session 1: Introduction and Structured Illumination Microscopy
11:00 – 11:15 Welcome
Erik Manders (UvA) and Sumio Eimori (Nikon)
11:15 – 11:45 Why microscopy is essential in an omics world
Hans Tanke ( Leiden University, Netherlands)
11:45 – 12:15 Structured illumination and confocal microscopy
Tony Wilson (Oxford University, United Kingdom)
12:15 – 12:45 Correlative SIM-TEM imaging in studies of higher-order chromatin folding
Igor Kireev (Moscow State University, Moscow, Russia)
12:45 – 14:00 Lunch

Session 2: Structured Illumination Microscopy
14:00 – 14:30 Deriving the resolution from the uncertainty principle
Ernst Stelzer (Goethe Universität Frankfurt am Main, Germany)
14:30 – 15:00 Beating the diffraction limit with Nikon Super Resolution Microscopes
Catherine Kitts (Nikon Europe BV, Amsterdam, Netherlands)
15:00 – 15.30 Multi-Focus Microscopy“
Sara Abrahamsson (The Rockefeller University, New York, USA)
15:30 – 16:00 Tea break

Session 3: Localization microscopy
16:00 – 16:30 Advances in superresolution microscopy: from developing probes to superresolution imaging in C. elegans“
Johan Hofkens (University Leuven, Leuven, Belgium)
16:30 – 17:00 Fast reversible photoconversion of fluorescent proteins for better resolution in time and space“
Alexander Mishin (Institute of Bioorganic Chemistry, Moscow, Russia)
17:00 Drinks in main hall of Science Park

Thursday, January 30, 2014 Session 4: Point-scanning super-resolution techniques
9:00 – 9:30 Optical Photon Reassignment Microscopy
Rainer Heintzmann (Institute of Photonic Technology, Jena, Germany)
9:30 – 10:00 Doubled resolution at 100 frames per second“
Andrew York (NIH, Bethesda, Maryland, USA)
10:00 – 10:30 Re-scan Confocal Microscopy“
Erik Manders (University of Amsterdam, Amsterdam, The Netherlands)
10:30 – 11:00 Coffee break

Session 5: Localization microscopy
11:00 – 11:30 Eight Years of Single Molecule Localization Microscopy
Markus Sauer (Universität Würzburg, Würzburg, Germany)
11:30 – 12:00 Making localization microscopy count
Sjoerd Stallinga (Technical University Delft, Delft, Netherlands)
12:00- 12:30 Image reconstruction for localization microscopy
Marten Postma (University of Amsterdam, Amsterdam, The Netherlands)
12.30- 13:45 Lunch

Session 6: Live-cell super-resolution
13:45 – 14:15 Light Sheet-based Fluorescence Microscopy (SPIM, DSLM, LSFM) for high spatio-temporal resolution
Ernst Stelzer (Goethe Universität Frankfurt am Main, Germany)
14:15 – 14:45 Nanoscopy and light sheet microscopy in immunology
Matthias Gunzer (University of Duisburg-Essen, Germany)
14:45 – 15:15 Cell type-specific STORM imaging in brain circuits
István Katona (Institute of Experimental Med., Budapest, Hungary)
15:15 – 15:45 Tea break

Session 7: STED-microscopy
15:45 – 16:15 STED nanoscopy combined with optical tweezers: a dynamics of proteins on densely covered DNA
Erwin Peterman (Free University, Amsterdam, Netherlands)
16:15 – 16:45 Beyond STED microscopy
Paolo Bianchini (Istituto Italiano di Tecnologia, Genova, Italy)
16:45 – 17:00 Closing session
Fred Brakenhoff and Erik Manders (University of Amsterdam, The Netherlands)